2 years ago

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This expands the position of Skp1 and its modifications in developmental regulation, and supports the model that O2 regulates its modification in cells. Cell development Cells had been harvested by centrifugation at four C, resuspended in PDF buff read more...



2 years ago

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aerogenes bacteria. The plating efficiency of cyst spores was 70%, very similar to that of spores collected from fruiting bodies on filters, which was 66%. Consequently, terminal cell differentiation occurred in radially symmetrical fash ion durin read more...



2 years ago

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Alternatively, they remained as prespore cells, primarily based on Western blot ana lysis exhibiting abundant expression on the spore coat pre cursors. Failure to sporulate was as a result of PhyA deficiency, since phyA cells complemented with ecm read more...



2 years ago

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Following 15 min, non bound spores were removed by aspir ation and washing with TBS. The monolayer was incu bated in four mg ml hemoglobin in TBS for five min, 1 ug ml mAb 83. 5 in 4 mg ml hemoglobin in TBS for one h, TBS, two ug ml Alexa 568 conj read more...



2 years ago

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In comparison, 5% O2 cells accu mulated considerable Skp1 within the position of the lower band. This band corresponds to unmodified Skp1 based mostly on reactivity with pAb UOK87. UOK87 pre ferentially binds unmodified Skp1 but exhibits weak re a read more...